Training in Induced Pluripotent Stem Cell Culture Techniques

Sunday, June 30, 2019 (All day)
Event Details: 

Courses run from January 2, 2019 through June 30, 2019 - Contact Yoav Gilad, gilad@uchicago.edu or Jonathan Burnett jburnett1@uchicago.edu for details.

Basic Course
Goal: To be able to receive well established iPSCs and culture them in your own lab.
Time estimate: 42 hours over 14 days, including one weekend (average of 3 hours a day)
Cost: $2280

Intermediate Course
Goal: To be able to receive iPSCs on feeder cells, transfer them to feeder free media and culture them in your own lab.
Time estimate: 72 hours over 24 days, including two weekends (average of 3 hours a day)
Cost: $5380

Advanced Course
Goal: To be able to reprogram iPSCs from somatic cells, transfer them to feeder free media and culture them in your own lab.
Time estimate: 105 hours over 35 days, including three weekends (average of 3 hours a day)
Cost: $8200

Differentiation Course (can be combined with any of the above courses)
Goal: To be able to differentiate iPSCs into cardiomyocytes. 
Time estimate: 33 hours over 11 days, including one weekend (average of 3 hours a day)
Cost: $3320

Mouse Embryonic Fibroblasts (MEFs)
Mouse embryonic fibroblasts (MEFs) are often used as "feeder cells" in human embryonic stem cell research. We estimate that 15 million MEF cells are required for the reprogramming of a single iPSC line. The core can provide high quality MEF cells (at least 90% viability) for researchers, at a cost of $225 for 15M cells (or $15 for each aliquot of 1M MEF cells).

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